Background: Biodentine is a calcium silicate based material which has shown to overcome the shortcomings of Mineral trioxide aggregate (MTA). The present study has assessed surface microhardness of biodentine using ethylenediaminetetraacetic acid (EDTA) and maleic acid. Materials & Methods: 60 Biodentine cylindrical shaped specimens were prepared and divided into 3 groups of 20 each. In Group I, the specimens were treated with 7% maleic acid (MA), in Group II with 17% EDTA and in Group III with 5 mL distilled water as control. The surface microhardness of the specimens were measured using Knoop hardness tester. Results: The mean Knoop hardness number (KHN) in group I was 64.7 KHN, in group II was 75.2 KHN and in group III was 120.3 KHN. The difference was significant (P< 0.05). Conclusion: Microhardness was reduced to the maximum level with maleic acid as compared to EDTA. Key words: Bio dentine, Ethylenediaminetetraacetic acid (EDTA), Maleic acid (MA)