Periodontitis causes inflammation and destruction of the periodontium resulting in progressive bone loss and pocket formation or recession of gingival. The ultimate goal of periodontal therapy is the regeneration of periodontium to restore lost form, function and esthetics. The aim of the present study was to evaluate the contribution of intramarrow penetration to the clinical and radiolographic outcomes of open flap debridement with platelet rich fibrin in the treatment of periodontal intrabony defects. A total of 20 sites in 10 subjects suffering from chronic periodontitis showing evidence of clinical probing depth ≥5mm with radiographic evidence of almost identical intrabony defects bilaterally were selected from amongst those reporting to the department of Periodontology, Punjab Government Dental College and Hospital, Amritsar. The sites were divided randomly into 2 groups. In group1, open flap debridement followed by placement of Platelet rich fibrin was done and in group 2, open flap debridement with intramarrow penetration followed by placement of platelet rich fibrin was done. All treated sites showed remarkable reduction in probing depth, clinical attachment gain and radiographic fill of periodontal defects. The combination of intramarrow penetration and platelet rich fibrin demonstrated better results in clinical as well as radiographic parameters than platelet rich fibrin alone. The present study has concluded positive clinical and radiographic outcomes following treatment with the combination of intramarrow penetration and PRF in intrabony defects, supporting a possible role in the future. Key words: Intra-bony defects, Fibrin