Background: The present study was planned was for assessing alteration in serum bilirubin levels in patients undergoing laparoscopic cholecystectomy. Materials & methods: The present study was planned was for assessing alteration in serum bilirubin levels in patients undergoing laparoscopic cholecystectomy. 50 patients undergoing laparoscopic cholecystectomy were enrolled. A detailed work up of all the patients enrolled in the study i.e detailed history, a thorough Clinical Examination was performed, followed by routine investigations including CBC (complete blood count), FBS (fasting blood sugar)/RBS (random blood sugar), PTI (prothrombin time index)/INR (international normalized ratio), RFTs (renal function tests), LFTs (liver function tests), Urine Routine, Serum Electrolytes, Viral Markers, Chest X Ray, Ultrasound Abdomen. The liver function tests were repeated on follow-up. All the results were analysed by SPSS software version 16.0. Results: Mean total bilirubin values during preoperative and postoperative 24 hours was 0.85 and 1.58 mg/dL respectively. Mean direct bilirubin values during preoperative and postoperative 24 hours was 0.35 and 0.52 mg/dL respectively. A significant rise in bilirubin values was seen postoperatively in patients undergoing laparoscopic cholecystectomy. Conclusion: Significant alteration in bilirubin levels occurs during laparoscopic cholecystectomy procedures. Key word: Bilirubin profile, Laparoscopic cholecystectomy