Background: Gingival fluid is composed mainly of blood electrolytes and organic molecules. The present study was conducted to assess the level of Neopterin in gingival crevicular fluid in healthy, chronic gingivitis and chronic periodontitis patients. Materials & Methods: This study was conducted on 78 subjects of both genders. Subjects were divided into 3 groups of 28 each. Group I comprised of healthy subjects, group II chronic gingivitis patients and group III chronic periodontitis patients. Parameters such as simplified oral hygiene index (OHI‑S), gingival index (GI), probing depth and clinical attachment level were recorded. Results: The mean OHI in group I was 0.45, in group II was 1.71 and in group III was 2.57. The difference was significant (P< 0.05). The mean gingival index in group I was 0.12, in group II was 1.14 and in group III was 1.84. The difference was significant (P< 0.05). The mean clinical attachment level in group I was 0.01, in group II was 1.04 and in group III was 4.25. The difference was significant (P< 0.05). Conclusion: Authors found that the neopterin levels in GCF are positively associated with periodontal disease. Key words: Neopterin, Gingivitis, Periodontitis.